An Interactive Annotated World Bibliography of Printed and Digital Works in the History of Medicine and the Life Sciences from Circa 2000 BCE to 2024 by Fielding H. Garrison (1870-1935), Leslie T. Morton (1907-2004), and Jeremy M. Norman (1945- ) Traditionally Known as “Garrison-Morton”

16061 entries, 14144 authors and 1947 subjects. Updated: December 10, 2024

Permanent Link for Entry #16617

  • 14294

In vivo imaging of membraine-associated glycans in developing Zebrafish.

Science, 320, 665-667, 2008.

The authors applied click chemistry to previously inaccessible biologic
environments. Towards that end, they used a modified azide and clicking it onto an alkyne but without using copper ions which are toxic to cells, they invent a reaction between azides and alkynes that reacts in a vigorous manner and totally without the help of copper. With this they invent a copper free click reaction called the “strain-promoted alkyne-azide cycloaddition’ or the ‘SPAAC’ reaction. By adding fluorophores (see Green Fluorescent Protein GM- 13564) to the reaction platform, they could
illuminate glycans in the cell surface and the emerging glycome of the zebrafish.

Order of authorship in the original publication: Laughlin, Baskin, et al, Bertozzi.

(Thanks to Juan Weiss for this reference and its interpretation.)



Subjects: Chemistry
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