An Interactive Annotated World Bibliography of Printed and Digital Works in the History of Medicine and the Life Sciences from Circa 2000 BCE to 2022 by Fielding H. Garrison (1870-1935), Leslie T. Morton (1907-2004), and Jeremy M. Norman (1945- ) Traditionally Known as “Garrison-Morton”

15961 entries, 13944 authors and 1935 subjects. Updated: March 22, 2024

CUI, Yiqiang

1 entries
  • 11863

Generation of gene-modified Cynomolgus monkey via CAS9/RNA-mediated gene targeting in one-cell embryos.

Cell, 156, 836-843, 2014.

Order of authorship in the original publication: Niu, Shen, Cui. The authors presented the first evidence that CRISPR can work in primates. Open Archive version available from Cell at this link. "Summary

"Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, we successfully achieve precise gene targeting in cynomolgus monkeys. We also show that this system enables simultaneous disruption of two target genes (Ppar-γ and Rag1) in one step, and no off-target mutagenesis was detected by comprehensive analysis. Thus, coinjection of one-cell-stage embryos with Cas9 mRNA and sgRNAs is an efficient and reliable approach for gene-modified cynomolgus monkey generation."

(Thanks to Juan Weiss for this reference and its interpretation.)

 



Subjects: BIOLOGY › MOLECULAR BIOLOGY › CRISPR Gene Editing